Name of instrument, m RealTime System composed of the msp and mrt modules. Country where designed/Manufactured/Reagents. The Abbott m System comprises the msp and mrz instruments, which are operated with separate System Control Center (SCC). J Clin Microbiol. Jun;47(6) doi: /JCM Epub Apr Performance of the Abbott real-time PCR assay using msp and .
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Comparison of HIV-1 Viral Load between Abbott m2000 and Roche COBAS TaqMan Methods
These two assays share three common features 1 Additional reduction in the lower limits of detection LOD of HIV-1 RNA from the k2000sp version; 2 New primers and probes designs for recognition of the different viral subtypes and circulating recombinant forms CRFs ; m2000xp 3 Reduction in hand-on time by configuring the test near to full automation.
Why request a quote through SelectScience? The Abbott m RealTime System provides automation from bar-coded laboratory tube through patient result, creating an efficient workflow for your laboratory. Furthermore, the input plasma volume is different between the two methods.
In this configuration, signal is not created by probe hydrolysis but by separation of the probe from quencher oligonucleotide.
Our data showed that these two methods are essentially indistinguishable with a correlation coefficient of 0. Following reverse j2000sp, the RNA: Be the first to review this product. The fluorescence counts were converted into VL measure by the analyzer Abbott, a ; Abbott, For examples, the Abbott method has been shown to have better detectability of xbbott subtypes Geelen et al.
Whether through new system software releases, innovative assay design or a constantly expanding mSystem menu, we are continuously evolving.
Differences in gene target, test sensitivity, input volume and their abilities to detect different HIV-1 subtypes could potentially explain some of the discordance. The Abbott m 0. Comparison of the mean VL with differences of the two methods using the Bland Altman plot showed rough symmetric distribution of the differences, indicating neither method is better than the other for measuring VL.
The difference between these two methods was Log10 0. This method has now become a standard method for monitoring HIV-infected patients on antiretroviral therapy in the United States. Secure We only pass your details on to trusted suppliers at your request. Can’t read the image?
m RealTime System from Abbott | SelectScience
The solid line in abbot box indicates the median value and the ends of the whiskers indicate the minimum and maximum values. Of ND Roche samples, 15 of them The extracted NA was mixed with the amplification reagents before amplification with the mrt Abbott, a ; Abbott, The Bland-Altman analysis was used for the analysis of the concordance of the two methods Bland and Altman, It is unclear at the moment the source of the discrepancies.
While the Roche method uses the standard TaqMan technology, a different and partially double stranded probe is introduced to the Abbott method Finan and Zhao, ; Johanson et al. This automated system uses magnetic microparticles-based reagents for the purification of nucleic acid from the samples.
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Using the TaqMan analyzer, the sample extraction volume protocol is 0. The general principle for NA extraction is similar to that of the Roche method, i. Review summary M Correa Posted on Dec 08 at 7: However, this factor should not be the major contributing factor because a recent surveillance study at the University of Maryland Medical Center showed that about Consequently, even though abbott methods are based on the same principle, due to the differences in their proprietary primer and probe designs, compatibility of these two methods has not been well documented.
This gene-target based amplification method is abgott on the determination of the threshold cycle CT when the amplified product is detected for the first time and the PCR is still in its exponential phase Ciotti et al. Detection by real-time technology used two probes of different lengths: The plot to the left represents the distribution of the VL from the Abbott method assay and the plot to the right represents the distribution of VL from the Roche assay.
Receive your quote directly from the manufacturer. Pharmaceutical Sciences Journals Ann Jose ankara escort. The Abbott method uses real-time platforms with fluorescence detection systems Abbott, b ; Scott et al.
Abbott Molecular is committed to molecular diagnostics. J Antivir Antiretrovir 2: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
This trend has lead to the development and approval of a number of diagnostic assays For reviews of these assays, see Constantine and Zhao, m2000wp The vertical axis represenst the VL differences the Abbott method minus the Roche method and the horizontal axis represenst the means of VL.